DNA-POLYMERASES ASSOCIATED WITH THE ADENOVIRUS TYPE-2 REPLICATION COMPLEX - EFFECT OF 2'-3'-DIDEOXYTHYMIDINE-5'-TRIPHOSPHATE ON VIRAL-DNA SYNTHESIS

An Adenovirus (Ad) DNA replication complex extracted from infected HeLa nuclei could be purified free of the bulk of intracellular DNA polymerase activity by sedimention in neutral sucrose gradients. However, the replication complex still retained some α and γ DNA polymerase activity. Since this complex is inhibited by 2′, 3′ dideoxythymidine-5′--triphosphate (ddTTP), an inhibitor of DNA polymerase γ, a functional role for this enzyme in Ad DNA replication is suggested. Similar inhibition by ddTTP in intact Ad infected nuclei and comparable inhibition of Ad DNA synthesis in whole cells by dideoxythymidine (ddThy) are consistent with a role for DNA polymerase γ. Uninfected HeLa nuclei or whole cells are not similarly inhibited by ddTTP or ddThy respectively. Such data does not rule out an additional functional role for other DNA polymerases, and recent experiments from this laboratory (1) suggest that DNA polymerase α is also involved in Ad DNA synthesis. © 1979 Information Retrieval Limited.