CALCIUM-SPECIFIC PRECIPITATION OF DENTIN PHOSPHOPROTEIN - NEW METHOD OF PURIFICATION AND THE SIGNIFICANCE FOR THE MECHANISM OF CALCIFICATION

被引:94
作者
KUBOKI, Y [1 ]
FUJISAWA, R [1 ]
AOYAMA, K [1 ]
SASAKI, S [1 ]
机构
[1] TOKYO MED & DENT UNIV,SCH DENT,DEPT BIOCHEM,TOKYO 113,JAPAN
关键词
D O I
10.1177/00220345790580092001
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Calcium, but not magnesium or strontium ions, specifically induce the precipitation of dentin phosphoprotein, which is reversibly solubilized by EDTA. This finding is very useful in purifying dentin phosphoprotein of either a free or matrix-bound type. Bovine dentin phosphoproteins, thus isolated, contain a minimal amount of contaminant protein, unlike previous preparations. © 1979, SAGE Publications. All rights reserved.
引用
收藏
页码:1926 / 1932
页数:7
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