ANTIGENIC AND ALLERGENIC CHARACTERIZATION OF THE ENZYMES ALCALASE AND SAVINASE BY CROSSED IMMUNOELECTROPHORESIS AND CROSSED RADIOIMMUNOELECTROPHORESIS

被引：6

作者：

ARLIAN, LG ^{[1
]}

VYSZENSKIMOHER, DL ^{[1
]}

MERSKI, JA ^{[1
]}

RITZ, HL ^{[1
]}

NUSAIR, TL ^{[1
]}

WILSON, ER ^{[1
]}

机构：

[1] PROCTER & GAMBLE CO,CINCINNATI,OH 45239

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY | 1990年 / 91卷 / 03期

关键词：

D O I：

10.1159/000235128

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Alcalase and savinase, produced by Bacillus species, are proteolytic enzymes that are used in laundry products and are known to cause respiratory allergy. Antigenic and allergenic characteristics of alcalase and savinase and their potential cross-reactivity were evaluated using crossed immunoelectrophoresis and crossed radioimmunoelectrophoresis. Alcalase exhibited two distinct antigens; one electropositive and one electronegative. The electropositive antigen exhibited some retrograde anodic mobility when coupled with antiserum components. Savinase exhibited one electropositive and two electronegative antigens. The antigens of the two enzymes were clearly different from each other, the three savinase antigens exhibiting greater electro-phoretic mobility than the two alcalase antigens. In crossed radioimmunoelectrophoresis studies, only the electropositive antigen of alcalase, its retrograde complex, and the electropositive antigen of savinase bound IgE from the sera of individuals who were skin test positive to one or both enzymes. No evidence of cross-reactivity was observed in heterologous and tandem crossed immunoelectrophoresis studies and heterologous microim-munodiffusion reactions. © 1990 S. Karger AG, Basel.

引用

页码：278 / 284

页数：7

共 13 条

[1] CROSS ANTIGENIC AND ALLERGENIC PROPERTIES OF THE HOUSE DUST MITE DERMATOPHAGOIDES-FARINAE AND THE STORAGE MITE TYROPHAGUS-PUTRESCENTIAE [J].