IMMOBILIZATION OF INVERTASE BY ENCAPSULATION IN POLYELECTROLYTE COMPLEXES

被引：43

作者：

MANSFELD, J

FORSTER, M

SCHELLENBERGER, A

DAUTZENBERG, H

机构：

[1] MARTIN LUTHER UNIV,DEPT BIOTECHNOL,O-04050 HALLE,GERMANY

[2] ACAD SCI GDR,INST POLYMER CHEM ERICH CORRENS,TELTOW,GERMANY

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1991年 / 13卷 / 03期

关键词：

IMMOBILIZATION; INVERTASE; ENCAPSULATION; POLYELECTROLYTE COMPLEXES; CELLULOSE SULFATE POLY(DIMETHYLDIALLYLAMMONIUM CHLORIDE); KINETICS; STABILITY;

D O I：

10.1016/0141-0229(91)90135-W

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Free and polystyrene-bound invertase from Saccharomyces cerevisiae were encapsulated within symplex membranes which were composed of cellulose sulfate as the polymeric anion and poly(dimethyldiallylammonium chloride) as the polymeric cation. The kinetics and the performance of the encapsulated enzyme preparations have been compared to the free enzyme employing the hydrolysis of sucrose. The pH and temperature optima were only slightly affected by the encapsulation. The kinetic constants, however, were changed by the encapsulation as a result of diffusional limitation. Encapsulated invertase showed a high storage stability and a high operational stability if low substrate concentrations were applied. The coimmobilization of invertase with living cells, which are not capable of utilizing sucrose, in the described capsules, opens many possibilities in fermentation technology.

引用

页码：240 / 244

页数：5

共 11 条

[1]

[Anonymous], 1973, CHEM KINETICS ENZYME

[2]

COMBES D, 1983, CARBOHYD RES, V177, P215

[3] EFFECT OF VISCOSITY ON SUCROSE HYDROLYSIS CATALYZED BY INVERTASE OBTAINED FROM S-CEREVISIAE [J].