CHARACTERIZATION OF A POLYGALACTURONASE GENE OF ASPERGILLUS-NIGER-RH5344

被引：25

作者：

RUTTKOWSKI, E ^{[1
]}

KHANH, NQ ^{[1
]}

WIENTJES, FJ ^{[1
]}

GOTTSCHALK, M ^{[1
]}

机构：

[1] ROHM GMBH,W-6100 DARMSTADT,GERMANY

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb00782.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned a gene encoding a polygalacturonase (PG) in the filamentous fungus Aspergillus niger RH5344. The structural gene comprises 1141 bp coding for 362 amino acids and the open reading frame is disrupted by one intron of 52 bp. Eukaryotic consensus sequences for transcription regulation are found only in deviated forms. The biological functionality of the isolated PG gene was established by retransformation in A. niger and Aspergillus awamori. In addition, we have found that the PG protein of A. niger shares significant similarities with PG proteins from tomato and Erwinia carotovora. Comparison of the three enzymes revealed a highly conserved region in their C-terminal region probably comprising the elements of substrate binding and the catalytic centre.

引用

页码：1353 / 1361

页数：9

共 41 条

[1] NUCLEOTIDE-SEQUENCE OF THE CLONED MESSENGER-RNA AND GENE OF THE ADP ATP CARRIER FROM NEUROSPORA-CRASSA [J].