THE EFFECTS OF ULTRAVIOLET-RADIATION ON WOUND-HEALING

The effects of prior ultraviolet light exposure on wound tensile strength and skin histology were evaluated in the hairless guinea pig model. Hairless guinea pigs (strain IAF/HA-HO) were irradiated with either UVA (80 J/cm2) or UVB (0.46 J/cm2) every other day for 16 weeks. Following cessation of treatment, a standard dorsal wound was made in each animal, allowed to heal, and mechanically tested to failure at 21 days. Serial 4 mm punch biopsies were obtained prior to the initial exposure and at 2, 4, 8, 12 and 16 weeks. Histological examination with haematoxylin and eosin, trichrome and elastin stains was performed. In comparison to the unexposed control group, would tensile strength was significantly less in the UVA- and UVB-irradiated animals. Histological examination revealed a marked endothelial swelling and eosinophilic infiltration in the irradiated groups. These results indicate that repeated exposure to even moderate doses of non-ionising radiation alters normal skin structure and adversely affects subsequent wound tensile strength in this model.