CYTOSOLIC CA-2+-INDUCED MODULATION OF ION SELECTIVITY AND AMILORIDE SENSITIVITY OF A CATION CHANNEL AND BETA AGONIST ACTION IN FETAL LUNG EPITHELIUM

The cytosolic Ca2+ concentration ([Ca2+]i) affects many cell functions, including the modulation of ion channel activity. In this study patch clamp experiments using primary cultures of fetal distal lung epithelium (FDLE) demonstrated that the elevation of [Ca2+]i modulated a 25pS amiloride-blockable non selective cation (NSC) channel's ion selectivity and sensitivity to amiloride. An elevation of [Ca2+]i from 0.1μM to 1mM both increased open probability (Po) and decreased the ratio of the premeability to Na to the permeability to K ( PNa PK) from 1.96±0.11 (SEM, n=6) to 0.88±0.04 (n=6). Within the range of [Ca2+]i from 0.1μM to 100μM amiloride (0.5μM) decreased Po, however amiloride (0.5μM) no longer affected Po of the NSC channel when [Ca2+]i was increased to 1mM under physiologic membrane potentials. A β adrenergic agonist (terbutaline, 10μM) increased Po in cellattached patches from almost 0 (Po<0.01; n=9) to 0.39±0.09 (n=9) and [Ca2+]i from 40±6nM (n=9) to more than 1μM. This suggested that amiloride-blockable NSC channel activity and ion permeability are modulated by changes in [Ca2+]i near physiologic membrane potentials and a β adrenergic agonist increases [Ca2+]i to more than 1μM (unlike other epithelial including adult alveolar cells) which is associated with activation the NSC channel. © 1992.