SAME-DAY BATCH MEASUREMENT OF GLYCINE BETAINE, CARNITINE, AND OTHER BETAINES IN BIOLOGICAL-MATERIAL

被引：65

作者：

LEVER, M ^{[1
]}

BASON, L ^{[1
]}

LEAVER, C ^{[1
]}

HAYMAN, CM ^{[1
]}

CHAMBERS, ST ^{[1
]}

机构：

[1] CHRISTCHURCH HOSP,DEPT INFECT DIS,CHRISTCHURCH,NEW ZEALAND

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 205卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0003-2697(92)90572-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycine betaine, carnitine, carnitine esters, butyrobetaine, and proline betaine (stachydrine) concentrations in biological materials can be reliably measured in 100-μl samples, with a detection limit below 1 μmol/liter. The procedure is suitable for batches of more than 30 specimens and it is possible to obtain a single result within 2 h. The betaines are extracted into an acetonitrile:methanol mixture, dried with anhydrous disodium hydrogen phosphate containing argentous oxide. The 4-bromophenacyl ester derivatives are formed using 4-bromophenacyl triflate as reagent, in the presence of solid magnesium oxide as base. The derivatives are separated by high-performance chromatography on a silica column, in a mixed partition and ion-exchange mode. © 1992.

引用

页码：14 / 21

页数：8

共 8 条

[1] CLINICAL-VARIETIES OF CARNITINE AND CARNITINE PALMITOYLTRANSFERASE DEFICIENCY [J].

ANGELINI, C ;

TREVISAN, C ;

ISAYA, G ;

PEGOLO, G ;

VERGANI, L .

CLINICAL BIOCHEMISTRY, 1987, 20 (01) :1-7

[2] THE ISOLATION OF L-STACHYDRINE FROM THE FRUIT OF CAPPARIS-TOMENTOSA [J].

CORNFORTH, JW ;

HENRY, AJ .

JOURNAL OF THE CHEMICAL SOCIETY, 1952, (FEB) :601-603

[3]

GULLANS SR, 1989, RENAL PHYSIOL BIOCH, V12, P191

[4] DERIVATIZATION OF CARBOXYLIC-ACIDS BY REACTION WITH 4'-BROMOPHENACYL TRIFLUOROMETHANESULFONATE PRIOR TO DETERMINATION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

INGALLS, ST ;

MINKLER, PE ;

HOPPEL, CL ;

NORDLANDER, JE .

JOURNAL OF CHROMATOGRAPHY, 1984, 299 (02) :365-376

[5] DETERMINATION OF CARNITINE, BUTYROBETAINE, AND BETAINE AS 4'-BROMOPHENACYL ESTER DERIVATIVES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

MINKLER, PE ;

INGALLS, ST ;

KORMOS, LS ;

WEIR, DE ;

HOPPEL, CL .

JOURNAL OF CHROMATOGRAPHY, 1984, 336 (02) :271-283

[6] CARNITINE - METABOLISM AND CLINICAL-CHEMISTRY [J].

SILIPRANDI, N ;

SARTORELLI, L ;

CIMAN, M ;

DILISA, F .

CLINICA CHIMICA ACTA, 1989, 183 (01) :3-11

[7]

WOLFF SD, 1990, ANNU REV PHYSIOL, V52, P727

[8] DETERMINATION OF CARNITINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING 9-ANTHRYLDIAZOMETHANE [J].

YOSHIDA, T ;

AETAKE, A ;

YAMAGUCHI, H ;

NIMURA, N ;

KINOSHITA, T .

JOURNAL OF CHROMATOGRAPHY, 1988, 445 (01) :175-182

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