ANTITHROMBOTIC EFFECT OF RECOMBINANT HUMAN SOLUBLE THROMBOMODULIN ON ENDOTOXIN-INDUCED DISSEMINATED INTRAVASCULAR COAGULATION IN RATS

被引：50

作者：

GONDA, Y ^{[1
]}

HIRATA, S ^{[1
]}

SAITOH, K ^{[1
]}

AOKI, Y ^{[1
]}

MOHRI, M ^{[1
]}

GOMI, K ^{[1
]}

SUGIHARA, T ^{[1
]}

KIYOTA, T ^{[1
]}

YAMAMOTO, S ^{[1
]}

ISHIDA, T ^{[1
]}

MARUYAMA, I ^{[1
]}

机构：

[1] KAGOSHIMA UNIV, SCH MED, DEPT CLIN LAB MED, KAGOSHIMA 890, JAPAN

来源：

THROMBOSIS RESEARCH | 1993年 / 71卷 / 04期

关键词：

THROMBOMODULIN; DIC; HEPARIN; ENDOTOXIN;

D O I：

10.1016/0049-3848(93)90201-X

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Thrombomodulin (TM) is an endothelial cell membrane glycoprotein which neutralizes thrombin procoagulant activity and accelerates the thrombin-catalyzed activation of protein C. We expressed recombinant human soluble TM (rhs-TM) in Chinese hamster ovary cells and compared the effects of rhs-TM and heparin on endotoxin-induced experimental disseminated intravascular coagulation (DIC) in rats. Experimental DIC was induced by a continuous intravenous infusion of endotoxin for four hours. rhs-TM or heparin was infused simultaneously with endotoxin. Treatment with rhs-TM significantly reversed the endotoxin-induced changes in the following parameters: platelet count, fibrinogen level and fibrinogen and fibrin degradation products. Furthermore, glomerular fibrin deposits elevated by endotoxin treatment were reduced by the rhs-TM administration. Heparin showed the similar effects to rhs-TM. Activated partial thromboplastin time (APTT) in rats receiving rhs-TM were slightly longer than APTT in endotoxin-treated rats, but rats receiving heparin had much more prolonged APTT. From these results, we concluded that rhs-TM may be useful for the clinical treatment of DIC while having only minor adverse effects on APTT.

引用

页码：325 / 335

页数：11

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