HUMAN PLACENTAL ALKALINE-PHOSPHATASE COVALENTLY IMMOBILIZED ON A CROSS-FLOW MICROFILTRATION POLYVINYLIDENE DIFLUORIDE MEMBRANE .2. ACTIVITY VS [SUBSTRATE] KINETIC-BEHAVIOR

被引：4

作者：

ROIG, MG ^{[1
]}

BELLO, JF ^{[1
]}

RODRIGUEZ, S ^{[1
]}

CACHAZA, JM ^{[1
]}

KENNEDY, JF ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,SCH CHEM,CHEM BIOACT CARBOHYDRATES & PROT RES LAB,BIRMINGHAM B15 2TT,ENGLAND

来源：

JOURNAL OF MOLECULAR CATALYSIS | 1994年 / 93卷 / 01期

关键词：

AFFINITY MEMBRANE; ALKALINE PHOSPHATASE; HUMAN PHOSPHATASE; IMMOBILIZED ENZYMES; KINETICS; MECHANISM; PLACENTAL ALKALINE PHOSPHATASE; POLYVINYLIDENE DIFLUORIDE;

D O I：

10.1016/0304-5102(94)00079-4

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Human placental alkaline phosphatase was chemically immobilized on a hydrophilic cross-flow microfiltration membrane made of polyvinylidene difluoride (PVDF) chemically labelled with 1-carbonylimidazole groups and characterized physicochemically (in Part I, J. Mol, Catal. (Vol. 93, 85-104)) The intrinsic kinetic behaviour (rate vs. substrate concentration) in the absence of diffusional constraints was analysed graphically and numerically, by non-linear regression and use of the F statistical test for model discrimination, postulating a minimum rational rate equation of 2:2 degree in substrate concentration. According to the results found, a mechanism for the catalytic kinetic action has been postulated.

引用

页码：105 / 117

页数：13

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