ANALYSIS OF OLIGO(DEOXYNUCLEOSIDE PHOSPHOROTHIOATE)S AND THEIR DIASTEREOMERIC COMPOSITION

被引：60

作者：

WILK, A ^{[1
]}

STEC, WJ ^{[1
]}

机构：

[1] POLISH ACAD SCI,CTR MOLEC & MACROMOLEC STUDIES,DEPT BIOORGAN CHEM,PL-90362 LODZ,POLAND

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 03期

关键词：

D O I：

10.1093/nar/23.3.530

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Short oligo(deoxynucleoside phosphorothioate)s were analyzed as a pool of individual diastereomeric species. The composition of such mixtures, determined by means of HPLC, indicates that consecutive couplings in commonly used phosphoramidite chemistry lead to increasing contents of the R(p) isomer. Methods of analysis and mathematical basis for diastereomeric composition are discussed. Data presented include all 16 possible combinations of nucleosides in dinucleotide phosphorothioates, as well as examples of trimers and tetramers.

引用

页码：530 / 534

页数：5

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