Luminescence immunoassay for allergen using alkaline phosphatase-conjugated IgE antibody with flow injection system

被引：10

作者：

Lim, TK

Nakamura, N

Matsunaga, T

机构：

来源：

DENKI KAGAKU | 1995年 / 63卷 / 12期

关键词：

flow injection; allergen; alkaline phosphatase-conjugated IgE antibody; liquid-phase luminescence immunoassay;

D O I：

10.5796/kogyobutsurikagaku.63.1154

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

An allergen detection system was developed based on a luminescence immunoassay with flow injection. Allergen and alkaline phosphatase-conjugated IgE antibody solution were mixed, incubated, and analyzed using a cation exchange column with a linear gradient of sodium chloride. The allergen-antibody complex was separated from free alkaline phosphatase-conjugated antibody. A linear allergen dose response curve was obtained between allergen concentration and the relative luminescence intensity. This simple, rapid and convenient immunoassay method can detect allergen continuously.

引用

页码：1154 / 1159

页数：6

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