THE PRODUCTION OF RECOMBINANT BETA-GALACTOSIDASE IN ESCHERICHIA-COLI IN YEAST EXTRACT ENRICHED MEDIUM

被引：30

作者：

LI, XL

ROBBINS, JW

TAYLOR, KB

机构：

[1] Department of Biochemistry, University of Alabama at Birmingham, Birmingham, 35294, Alabama

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1990年 / 5卷 / 2-3期

关键词：

Beta-galactosidase; Fermentation; Recombinant E. coli;

D O I：

10.1007/BF01573857

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The productivity of Escherichia coli biomass and recombinant beta-galactosidase was increased in Luria broth (LB) enriched with yeast extract. In flask culture under conditions of LB limitation, yeast extract suplementation gave the highest biomass (strain HB101/pRW756) stimulation per unit of component added compared with supplementation by various amounts of amino acids, vitamins, minerals, purines/pyrimidines, tryptone, casamino acids, casein peptone or gelatin peptone. The biomass production of E. coli HB101/pRW756, XL-1 blue/puc118, XL-1 Blue FF/puc118 and TB-1/p1034 cells was stimulated in fermentor-scale experiments with additional yeast extract in LB. Total beta-galactosidase production from plasmid genes in fermentor-scale experiments was increased 105.4% in XL-1 blue/puc118 cells, 365.5% in XL-1 blue FF/puc118 cells and 421.4% in TB-1/p1034 cells by 0.5%, 1% and 1% weight per volume of additional yeast extract in LB, respectively. Depending on different strains, the increase of the enzyme production was obtained either by increased biomass, or the combination of enhanced gene expression and increased biomass. Neither the biomass nor beta-galactosidase production was stimulated in N4830/p1034 cells by the increase in yeast extract concentration in the medium. © 1990 Society for Industrial Microbiology.

引用

页码：85 / 93

页数：9

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