SIMULTANEOUS DETERMINATION OF CEPHRADINE AND CEPHALEXIN IN SERUM BY DERIVATIVE SYNCHRONOUS FLUORESCENCE SPECTROSCOPY

被引：12

作者：

IZQUIERDO, P

GUTIERREZ, MC

GOMEZHENS, A

PEREZBENDITO, D

机构：

[1] Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba

来源：

ANALYTICAL LETTERS | 1990年 / 23卷 / 03期

关键词：

cephalexin; Cephradine; derivative-synchronous fluorimetry; serum;

D O I：

10.1080/00032719008052459

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The second-derivative synchronous fluorescence spectroscopic technique was applied to the simultaneous assay of cephradine and cephalexin in serum in developing a method based on the formation of the corresponding hydrolysis products of these compounds by treatment in an alkaline medium. Both the conventional and the synchronous fluorescence spectra of these hydrolysis products were completely overlapped, making it impossible to resolve the mixture. However, an appropriate selection of the instrumental variables affecting the second-derivative synchronous fluorescence spectra allowed the simultaneous determination of both compounds in a single scan. The calibration graphs run. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：487 / 505

页数：19

共 19 条

[1]

AIKAWA R, 1976, CHEM PHARM BULL, V24, P2350

[2] FLUORIMETRIC DETERMINATION OF CEFUROXIME IN BODY-FLUIDS [J].