ISOLATION OF A NAD =3-BETA(ALPHA)-HYDROXY-5-BETA-ANDROSTANE DEHYDROGENASE FROM RAT-LIVER MICROSOMES

被引：7

作者：

GOLF, SW

GRAEF, V

机构：

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1979年 / 10卷 / 02期

关键词：

D O I：

10.1016/0022-4731(79)90236-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A rat liver microsomal NAD:3β(α)-hydroxy-5β-androstane dehydrogenase was solubilized by the detergent Lubrol WX and purified by DEAE-cellulose chromatography and affinity chromatography. The homogeneous preparation (disc gel electrophoresis) catalyzed the reduction of 17β-hydroxy-5β-androstan-3-one to the corresponding 3α- and 3β-hydroxysteroids with NADH as hydrogen donor. The 3-oxo group of 17β-hydroxy-5α-androstane-3-one and 5β-pregnane-3,20-dione was not reduced. The purified enzyme preparation had a specific activity of 2.84 μmol 3β-hydroxysteroid formed/min mg and 1.61μmol 3α-hydroxysteroid formed/min mg. Phosphatidylcholine micelles (20mg/mg protein) were essential for activity. Molecular weight determination by Sephadex G-200 gave a value of 56,200. © 1979.

引用

页码：201 / 205

页数：5

共 18 条

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