VERSATILE, HIGH-RESOLUTION THIN-LAYER CHROMATOGRAPHIC SYSTEM FOR THE ANALYTICAL AND PREPARATIVE SEPARATION OF COMPLEXES AND AMINO-ACID LIGANDS

An effective thin-layer chromatographic (TLC) technique has been developed for the separation of cobalt(III) geometrical isomers and diastereomers and various derivatized amino acid and peptide ligands. A single solvent system containing isopropyl alcohol and a triethylammonium bicarbonate buffer is employed to achieve both analytical and preparative separations. The technique is more rapid and sometimes exhibits better resolution than conventional column ion-exchange chromatography. One-step purifications of compounds which have required tedious purification procedures have also been achieved. Isomers possessing aromatic functional groups which strongly adsorbed to the polystyrene matrix of ion-exchange resins can be separated easily by this TLC method. © 1979, American Chemical Society. All rights reserved.