THE CLASS-3 OUTER-MEMBRANE PROTEIN (PORB) OF NEISSERIA-MENINGITIDIS - GENE SEQUENCE AND HOMOLOGY TO THE GONOCOCCAL PORIN PIA

被引：21

作者：

WOLFF, K ^{[1
]}

STERN, A ^{[1
]}

机构：

[1] BOEHRINGER MANNHEIM GMBH,DEPT GENET,BB-GC,NONNENWALD 2,W-8122 PENZBERG,GERMANY

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 83卷 / 02期

关键词：

NEISSERIA-MENINGITIDIS; CLASS-3; PROTEIN; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0378-1097(91)90351-A

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The class 3 protein (PorB) is an important component of the meningococcal outer membrane. The structural gene (porB) encoding the class 3 protein has been cloned using primers suitable for the amplification of the corresponding chromosomal fragment by the polymerase chain reaction (PCR). The complete nucleotide sequence was determined and predicts a mature protein of 310 amino acids, preceded by a signal peptide of 19 residues. The predicted protein sequence of the class 3 protein exhibits essential structural homology to the gonococcal porin PIA. The class 3 protein encoding gene was expressed in Escherichia coli under the control of an inducible promotor.

引用

页码：179 / 185

页数：7

共 22 条

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