MORPHOLOGICAL AND FUNCTIONAL-CHARACTERIZATION OF A HORMONALLY INDUCED AIMS/GRXII CELL-LINE

The AIMS/GRXII cell line is a product of the line of research our laboratory is pursuing to understand the response of target cells to sustained hormonal stimulation, a situation simulating the one that brings about in vivo tumorigenesis. The cell line was derived from goat ovarian granulosa cells subjected to luteinizing hormone stress (high doses repeated). It was found to be contact-inhibited, nontumorigenic and secreted progesterone. The majority of cells at any given passage showed hypodiploidy. The cell line required hormonal support till passage 8 after which it was hormone-independent. Thus, the system offers a phase-wise development of the cell line which might be useful in cancer research, particularly to understand the mechanisms of cell transformation at the cellular and molecular level and possible hormone (onco)gene nexus in cell proliferation.