TRICHOBILHARZIA-OCELLATA IN LYMNAEA-STAGNALIS - A FLOW CYTOMETRIC APPROACH TO STUDY ITS EFFECTS ON HEMOCYTES

被引：12

作者：

AMEN, RI

ATEN, JA

BAGGEN, JMC

MEULEMAN, EA

DELANGEDEKLERK, ESM

SMINIA, T

机构：

[1] FREE UNIV AMSTERDAM,FAC MED,DEPT THEORY MED EPIDEMIOL & BIOSTAT,1081 BT AMSTERDAM,NETHERLANDS

[2] FREE UNIV AMSTERDAM,FAC MED,DEPT HISTOL,1081 BT AMSTERDAM,NETHERLANDS

[3] UNIV AMSTERDAM,DEPT RADIOBIOL,AMSTERDAM,NETHERLANDS

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 1992年 / 59卷 / 01期

关键词：

LYMNAEA-STAGNALIS; TRICHOBILHARZIA-OCELLATA; SCHISTOSOMES; SNAILS; HEMOCYTES; FLOW CYTOMETRY;

D O I：

10.1016/0022-2011(92)90117-M

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Flow cytometric analysis was performed on hemocytes in suspension derived from individual Lymnaea stagnalis. The distribution of cell sizes within the hemocyte population was comparable in all 40 specimens studied. The size distribution of circulating hemocytes is unimodal and continuous, with no discrete subpopulations, and is not affected by age or by infection with Trichobilharzia ocellata. Flow cytometry proved to be a very useful technique in analysis of hemocyte populations in snails and anti-hemocyte monoclonal antibodies can be employed in these studies. The use of individual instead of pooled hemolymph samples in studying hemocyte populations of molluscs is stressed. © 1992.

引用

页码：95 / 98

页数：4

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DIKKEBOOM R, 1985, IMMUNOLOGY, V55, P547

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