CONSTRUCTION OF ESCHERICHIA-COLI CLOSTRIDIUM-ACETOBUTYLICUM SHUTTLE VECTORS AND TRANSFORMATION OF CLOSTRIDIUM-ACETOBUTYLICUM STRAINS

被引：28

作者：

LEE, SY

BENNETT, GN

PAPOUTSAKIS, ET

机构：

[1] NORTHWESTERN UNIV,DEPT CHEM ENGN,EVANSTON,IL 60208

[2] RICE UNIV,DEPT BIOCHEM & CELL BIOL,HOUSTON,TX 77251

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 05期

关键词：

D O I：

10.1007/BF01021259

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several E. coli-C. acetobutylicum shuttle vectors were constructed and used to transform two C. acetobutylicum strains ATCC 824 and NCIMB 8052. Other than pSYL2, none of these vectors were able to transform ATCC 824 due to the presence of a restriction system. However, all of them could transform NCIMB 8052 with efficiencies of 8 x 10(2) - 6 x 10(3) transformants per mu-g DNA.

引用

页码：427 / 432

页数：6

共 19 条

[1] CONSTRUCTION OF PLASMID VECTORS FOR THE DETECTION OF STREPTOCOCCAL PROMOTERS [J].