DIRECT EXTRACTION AND PURIFICATION OF RIBOSOMAL-RNA FOR ECOLOGICAL-STUDIES

被引：86

作者：

MORAN, MA

TORSVIK, VL

TORSVIK, T

HODSON, RE

机构：

[1] UNIV BERGEN,DEPT MICROBIOL & PLANT PHYSIOL,N-5007 BERGEN,NORWAY

[2] UNIV GEORGIA,DEPT MARINE SCI,ATHENS,GA 30602

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 03期

关键词：

D O I：

10.1128/AEM.59.3.915-918.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microgram quantities of rRNA were recovered from natural microbial communities in sediment, soil, and water with a lysozyme-hot phenol direct extraction method. Gel filtration with Sephadex G-75 spun columns readily removed humic-like contaminants without any measurable loss of rRNA and rendered RNA extracts of sufficient quality for molecular procedures.

引用

页码：915 / 918

页数：4

共 19 条

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