EXPRESSION OF THE HERPES-SIMPLEX VIRUS THYMIDINE KINASE GENE IN SACCHAROMYCES-CEREVISIAE

被引：67

作者：

MCNEIL, JB

FRIESEN, JD

机构：

来源：

MOLECULAR AND GENERAL GENETICS | 1981年 / 184卷 / 03期

关键词：

D O I：

10.1007/BF00352510

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast plasmids were constructed that carry the herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) gene which is functionally expressed in S. cerevisiae. The expression of the TK gene apparently is due to transcriptional read-through from a yeast promoter that lies on the 3'' side of the HIS3 gene. The TK+ yeast processes in vitro thymidine kinase activity which is absent in the original yeast strain. Yeast strains auxotrophic for thymidine monophosphate (dTMP) (tmp1) can grown on thymidine-containing medium after transformation with these plasmids. Tmp+, TK+ S. cerevisiae whose de novo synthesis of dTMP is inhibited with amethopterin plus sulfanilamide is also capable of growth in thymidine. S. cerevisiae transformed with such plasmids is capable of incorporating thymidine and bromodeoxyuridine into DNA.

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页码：386 / 393

页数：8

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