TRANSPLANTED BETA-CELL RESPONSE TO INCREASED METABOLIC DEMAND - CHANGES IN BETA-CELL REPLICATION AND MASS

We determined the capacity of transplanted beta cells to modify their replication and mass when stimulated by changes in metabolic demand. Five groups of Lewis rats were studied: group 1 (Tx-Px) had a 95% pancreatectomy 14 d after transplantation of 500 islets; group 2 (Px-Tx) had a 95% pancreatectomy 14 d before transplantation of 500 islets; group 3 (Tx) was transplanted with 500 islets; group 4 (Px) had a 95% pancreatectomy; and group 5 (normal) was neither transplanted nor pancreatectomized. Blood glucose was normal in Tx-Px and Tx groups at all times. Px-Tx and Pr groups developed severe hyperglycemia after pancreatectomy that was corrected in Px-Tx group in 83% of rats 28 d after transplantation. Replication of transplanted beta cells increased in Tx-Px (1.15+/-0.12%) and Px-Tx (0.85+/-0.12%) groups, but not in Tx group (0.64+/-0.07%) compared with normal pancreatic beta cells (0.38+/-0.05%) (P < 0.001). Mean beta cell size increased in Tx-Px (311+/-14 mu m(2)) and Px-Tx (328+/-13 mu m(2)) groups compared with Tx (252+/-12 mu m(2)) and normal (239+/-9 mu m(2)) groups (P ( 0.001). Transplanted beta cell mass increased in Tx-Px (1.87+/-0.51 mg) and Px-Tx (1.55+/-0.21 mg) groups compared with Tx group (0.78+/-0.17 mg) (P < 0.05). In summary, changes in transplanted beta cells prevented the development of hyperglycemia in Tx-Px rats. Transplanted beta cells responded to increased metabolic demand increasing their beta cell mass.