SPECTROPHOTOMETRIC DETERMINATION OF TRIAMTERENE USING SOME ACID DYES

Two acid dye reagents have been utilized for spectrophotometric determination of triamterene in pure form and in pharmaceutical preparations. The dyes used are bromophenol blue (B.P.B), and bromothymol blue (B.T.B). They form a chloroform-soluble, coloured ion association complex with triamterene, at pH 3.4 and 3.2 using B.P.B and B.T.B,respectively. The formed complex could be extracted and measured spectrophotometrically at 417 nm The molar ratio of the formed drug-dye ion for both dyes. association complex is found to be 1:1 for both dyes used, as deduced by applying Job's method. Linearity was obtained using concentration ranges of 2.5-10 mu g. ml(-1) and 2.5-15 mu g.ml(-1) of triamterene with B.P.B. and B.T.B. respectively. The validity of the procedure was assessed by applying the standard addition technique. Results obtained by using the proposed procedure were statistically analysed and compared with those obtained by adopting the pharmacopoeial method.