SEPARATION AND PARTIAL-PURIFICATION OF 1,3-BETA-GLUCAN AND 1,4-BETA-GLUCAN SYNTHASES FROM SAPROLEGNIA

被引：26

作者：

BULONE, V ^{[1
]}

GIRARD, V ^{[1
]}

FEVRE, M ^{[1
]}

机构：

[1] UNIV LYON 1,CTR GENET MOLEC & CELLULAIRE,BIOL CELLULAIRE FONG LAB,CNRS,F-69622 VILLEURBANNE,FRANCE

来源：

PLANT PHYSIOLOGY | 1990年 / 94卷 / 04期

关键词：

D O I：

10.1104/pp.94.4.1748

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Enriched 1,3-beta-glucan and 1,4-beta-glucan synthase fractions from the fungus Saprolegnia were isolated by rate zonal centrifugation on glycerol gradient. Purification was improved by entrapment of the enzymes in their reaction product, i.e. microfibrillar glucans. 1,3-beta-Glucan syntheases were separated from 1,4-beta-glucan synthases following resuspension of entrapped enzymes. Sodium dodecylsulfate-polyacrylamide gel electrophoresis indicated that 1,3-beta-g lucan and 1,4-beta-glucan synthases may have a different polypeptide composition because they were enriched for different protein subunits (34, 48, an d 50 kD for the 1,3-beta-glucan synthase and 60 kD for the 1,4-beta-glucan synthase).

引用

页码：1748 / 1755

页数：8

共 22 条

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