SIMPLIFIED SOLID-PHASE IMMUNOBEAD ASSAY FOR DETECTION OF CIGUATOXIN AND RELATED POLYETHERS

被引：24

作者：

HOKAMA, Y

机构：

[1] Department of Pathology, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii

来源：

JOURNAL OF CLINICAL LABORATORY ANALYSIS | 1990年 / 4卷 / 03期

关键词：

colored latex; immunoassay; ciguatoxin; Marine toxins; toxic fish;

D O I：

10.1002/jcla.1860040313

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

This study examined the development of a highly simplified solid‐phase colored latex immunobead assay for the detection of ciguatoxin and related polyethers. This procedure was compared with the stick enzyme immunoassay previously reported. Chi‐square analysis of two separate experiments on 153 and 283 fish of various species gave χ2 values of p < 0.001 and p < 0.005, respectively Agreement between the two procedures with 26 fish implicated in ciguatera poisoning was 100%. A preliminary assessment in the field showed encouraging results. The procedure appears to be simple and applicable to field use. Furthermore, this procedure should be applicable to other antibody‐antigen detections, especially low dalton determinations. Copyright © 1990 Wiley Periodicals, Inc., A Wiley Company

引用

页码：213 / 217

页数：5

共 17 条

[1]

BADEN DG, 1985, TOXIC DINOFLAGELLATE, P363

[2]

BAGNIS RA, 1987, PROGR VENOM TOXIN RE, P272

[3] CIGUATERA POISONING - CLINICAL AND IMMUNOLOGICAL ASPECTS [J].