REFILLING OF ENDOTHELIAL CALCIUM STORES WITHOUT BYPASSING THE CYTOSOL

被引：13

作者：

LUCKHOFF, A

BUSSE, R

机构：

[1] Institute of Applied Physiology, University of Freiburg, D-7800 Freiburg i.Br.

来源：

FEBS LETTERS | 1990年 / 276卷 / 1-2期

关键词：

ENDOTHELIAL CELL; INTRACELLULAR CA-2+ STORE; CALCIUM UPTAKE INHIBITION; 2,5-DI(TERT-BUTYL)-1,4-BENZOHYDROQUINONE;

D O I：

10.1016/0014-5793(90)80519-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study was undertaken to define the route of Ca2+ used for refilling of intracellular Ca2+ stores in endothelial cells. Ca2+ stores, after emptying with bradykinin in Ca2+ free solution and termination of the stimulation with the bradykinin antagonist, Hoe 140, were allowed to refill by addition of Ca2+. Refilling was prevented by 2,5-di(tert-butyl)-1,4-benzohydroquinone (BuBHQ), and inhibitor of microsomal Ca2+ sequestration. BuBHQ induced large increases in the cytosolic Ca2+ concentration during the refilling phase. This finding is not compatible with a model proposing Ca2+ uptake into the stores directly from the extracellular space but provides evidence for uptake from the cytosolic compartment.

引用

页码：108 / 110

页数：3

共 17 条

[1]

DERIAN CK, 1986, J BIOL CHEM, V261, P3831

[2]

GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440

[3] INFLUX OF BIVALENT-CATIONS CAN BE INDEPENDENT OF RECEPTOR STIMULATION IN HUMAN-ENDOTHELIAL CELLS [J].