POLY(VINYLIDENE DIFLUORIDE) MEMBRANES AS THE INTERFACE BETWEEN LASER-DESORPTION MASS-SPECTROMETRY, GEL-ELECTROPHORESIS, AND IN-SITU PROTEOLYSIS

被引：123

作者：

VESTLING, MM

FENSELAU, C

机构：

[1] Chemistry and Biochemistry Department, University of Maryland Baltimore County, Baltimore

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 04期

关键词：

D O I：

10.1021/ac00076a009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A 337-nm laser has been used to desorb and ionize proteins from poly(vinylidene difluoride) membranes. Proteins separated by electrophoresis and electroblotted onto 0.5 X 7 cm membranes are located by scanning laser desorption, and their molecular weights are determined by mass spectrometry. Overlapping protein bands are easily deconvoluted. Conditions have been developed to carry out trypsin proteolysis of protein bands directly on the membrane. The same membrane is then reintroduced into the mass spectrometer, and peptide products are mapped by matrix-assisted laser desorption.

引用

页码：471 / 477

页数：7

共 33 条

[1] INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY ONE-DIMENSIONAL OR TWO-DIMENSIONAL GEL-ELECTROPHORESIS AFTER INSITU PROTEASE DIGESTION ON NITROCELLULOSE [J].