A RAPID IMMUNOSTAINING METHOD FOR FROZEN-SECTIONS

被引：52

作者：

CHILOSI, M ^{[1
]}

LESTANI, M ^{[1
]}

PEDRON, S ^{[1
]}

MONTAGNA, L ^{[1
]}

BENEDETTI, A ^{[1
]}

PIZZOLO, G ^{[1
]}

MENESTRINA, F ^{[1
]}

机构：

[1] UNIV VERONA,DEPT HEMATOL,I-37100 VERONA,ITALY

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1994年 / 69卷 / 04期

关键词：

IMMUNOHISTOCHEMISTRY; FROZEN SECTIONS; MICROWAVE; EPOS;

D O I：

10.3109/10520299409106292

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple and rapid one-step method for demonstrating immunohistochemical markers (leukocyte common antigen, cytokeratin, etc.) is described, which can help define the nature of poorly differentiated neoplasms for diagnosis using frozen section. Microwave irradiation was used to speed immunohistochemical analysis using ''Enhanced Polymer One-step Staining'' (EPOS) reagents on cryostat sections from a variety of pathologic samples. Reproducible results were obtained using EPOS reagents for leukocyte common antigen and cytokeratin. The overall procedure takes less than 10 min and can be completed during surgery.

引用

页码：235 / 239

页数：5

共 13 条

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