CO-TRANSCRIPTION OF ORF25 AND COXIII IN RICE MITOCHONDRIA

被引：26

作者：

LIU, AW

NARAYANAN, KK

ANDRE, CP

KALEIKAU, EK

WALBOT, V

机构：

[1] Department of Biological Sciences, Stanford University, Stanford, 94305, CA

来源：

CURRENT GENETICS | 1992年 / 21卷 / 06期

关键词：

MITOCHONDRIA; CO-TRANSCRIPTION; ORF25;

D O I：

10.1007/BF00351661

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Southern hybridization analysis using homologous maize probes indicated that orf25 and coxIII are closely linked in the mitochondrial genome of rice (Oryza sativa) cultivar IR36. The two coding regions were found on the same 5.1 kb BamHI fragment, and this fragment was cloned, mapped and partially sequenced. Using probes for each gene derived from the rice clone, a 2.4 kb dicistronic mRNA transcript was found containing both orf25 and coxIII coding regions. Multiple 5' ends were identified by primer extension analysis and a double stem/loop structure was mapped to the 3' end. The orf25 coding region shares > 85% identity with orf25 sequences from maize, tobacco and wheat, suggesting that orf25 may code for a conserved protein product.

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页码：507 / 513

页数：7

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