Production of alpha-Keto Acids Part I. Immobilized Cells of Trigonopsis variabilis Containing D-Amino Acid Oxidase

被引：77

作者：

Brodelius, Peter ^{[1
]}

Nilsson, Kjell ^{[1
]}

Mosbach, Klaus ^{[1
]}

机构：

[1] Univ Lund, Ctr Chem, S-22007 Lund, Sweden

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1981年 / 6卷 / 04期

关键词：

Production of alpha-keto acids; alpha-keto acids; production of; immobilized cells; in the production of alpha-keto acids; Trigonopsis variabilis; D-amino acid oxidase; in alpha-keto acid production; oxidase; D-amino acid; manganese oxide; co-immobilized with whole cells; liquid chromatography; assay of D-amino acid oxidase activity; chromatography; liquid; of D-amino acid oxidase activity;

D O I：

10.1007/BF02798280

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Whole cells of Trigonopsis variabilis were immobilized by entrapment in Ca2+-alginate and used for the production of alpha-keto acids from the corresponding D-amino acids. The D-amino acid oxidase within the immobilized cells has a broad substrate specificity. Hydrogen peroxide formed in the enzymatic reaction was efficiently hydrolyzed by manganese oxide co-immobilized with the cells. The amino acid oxidase activity was assayed with a new method based on reversed-phase HPLC. Oxygen requirements, bead size, concentration of cells in the beads, flow rate, and other factors were investigated in a "trickle-bed" reactor.

引用

页码：293 / 307

页数：15

共 17 条

[1] PURIFICATION OF D-AMINO-ACID OXIDASE FROM TRIGONOPSIS-VARIABILIS
BERG, CP
RODDEN, FA
[J]. ANALYTICAL BIOCHEMISTRY, 1976, 71 (01) : 214 - 222
[2] BERGSTRO.J, 1972, NUTR METAB, V14, P162
[3] Bouin J C, 1976, Methods Enzymol, V44, P478
[4] BRODELIUS P, 1980, ENZYME ENG, V5, P383
[5] Brodelius P., 1978, ADV BIOCHEM ENG, V10, P75
[6] ENZYME THERMISTOR DETERMINATION OF GLUCOSE IN SERUM USING IMMOBILIZED GLUCOSE OXIDASE
DANIELSSON, B
GADD, K
MATTIASSON, B
MOSBACH, K
[J]. CLINICA CHIMICA ACTA, 1977, 81 (02) : 163 - 175
[7] Fink D. J., 1977, 3 US USSR C DEV METH
[8] Fink D. J., 1976, ADV ENZYME ENG, V2, P79
[9] Goldstein L, 1976, Methods Enzymol, V44, P397
[10] KATCHALSKI E, 1971, ADV ENZYMOL RAMB, V34, P445

← 1 2 →