SITE-DIRECTED MUTAGENESIS OF LYS(600) IN PHOSPHOENOLPYRUVATE CARBOXYLASE OF FLAVERIA-TRINERVIA - ITS ROLES IN CATALYTIC AND REGULATORY FUNCTIONS

被引：16

作者：

GAO, Y ^{[1
]}

WOO, KC ^{[1
]}

机构：

[1] NO TERR UNIV, FAC SCI, SCH BIOL SCI, CASUARINA, NT 0909, AUSTRALIA

来源：

FEBS LETTERS | 1995年 / 375卷 / 1-2期

关键词：

PHOSPHOENOLPYRUVATE CARBOXYLASE; LYSINE; SUBSTRATE-BINDING SITE; FLAVERIA TRINERVIA;

D O I：

10.1016/0014-5793(95)01189-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphoenolpyruvate carboxylases from various organisms contain two conserved lysine residues, In the C-4 dicot Flaveria trinervia, one of these residues is Lys(600), Converting this Lys(600) to Arg(600) or Thr(600) mainly increased the K-m values and but had minimal effect on the V-max, The K-m for PEP, Mg2+ increased by up to 3-fold in Arg(600) and Thr(600) but the K-m (HCO3-) increased 9-fold in Thr(600), suggesting that Lys(600) might be associated with bicarbonate-binding, This lysine was not obligatory for enzyme activity although the wild-type protein showed higher activity at physiological pH and was less inhibited by malate than the two mutants.

引用

页码：95 / 98

页数：4

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