TIME-RESOLVED FLUORESCENCE ANISOTROPY STUDIES ON THE INTERACTION OF BIOLOGICALLY-ACTIVE POLYCATIONS WITH PHOSPHOLIPID-MEMBRANES

被引:11
作者
IKEDA, T
LEE, B
YAMAGUCHI, H
TAZUKE, S
机构
[1] Photochemical Process Division, Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Midori-ku, Yokohama, Nagatsuta
关键词
Fluorescence anisotropy; Phospholipid membrane; Polycation-membrane interaction; Polycationic biocide;
D O I
10.1016/0005-2736(90)90384-Z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Interaction of a polymeric in-chain quaternary ammonium salt with phospholipid bilayer membranes was studied by time-resolved fluorescence spectroscopy with the aid of a picosecond time-rcorrelated single photon counting system (instrument response function, 70 ps FWHM). Particular attention was paid to a phenomenon of polycation-induced fluidization of the membranes, which was well evaluated by time-resolved fluorescence anisotropy measurements. The fluorescence anisotropy, r(t), of 1,6-diphenyl-1,3,5-hexatriene (DPH) embedded in the membranes was analyzed based on the wobbling-in-cone model. Strong interaction was observed between the polycation and a negatively charged phosphatidic acid membrane as demonstrated by a large decrease of residual polarization value on adding the polycation to the acidic phospholipid dispersion. This means that the cone angle of the wobbling-in-cone motion of the DPH molecule increases by the addition of the polycaton, indicative of the polycation-induced fluidization of the acidic membrane. On the other hand, r(t) of DPH embedded in a zwitterionic phosphatidylcholine membrane was not affected significantly by the addition of the polycation, which is presumably due to non-binding of the polycation to the zwitterionic membrane. Our results clearly indicate that the polycationic disinfectant interacts strongly with negatively charged membranes, inducing fluidization of the membranes. © 1990.
引用
收藏
页码:56 / 62
页数:7
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