POLYOXIN D, A COMPETITIVE INHIBITOR OF UDP-N-ACETYLGLUCOSAMINE - CHITIN N-ACETYLGLUCOSAMINYLTRANSFERASE IN NEUROSPORA CRASSA

被引：98

作者：

ENDO, A

MISATO, T

机构：

[1] Central Research Laboratories, Sankyo Company, Ltd., Shinagawa-ku, Tokyo

[2] Institute of Physical and Chemical Research Yamato-machi, Saitama

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 37卷 / 04期

关键词：

D O I：

10.1016/0006-291X(69)90870-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The antibiotic polyoxin D inhibited the incorporation of glucosamine-14C into cell wall chitin in Neurospora crassa. In addition, it caused the accumulation of labeled UDP-N-acetylglucosamine (UDP-GlcNAc) under the same conditions, indicating an inhibition in the UDP-GlcNAc: chitin N-acetylglucosaminyltransferase (chitin synthetase) reaction. Kinetic studies of the cell free system showed that polyoxin D strongly inhibited the incorporation of N-acetylglucosamine from UDP-GlcNAc into chitin. The relation between polyoxin D and UDP-GlcNAc was a competitive one. The Km for UDP-GlcNAc and the Ki for polyoxin D was 1.43 x 10-3M and 1.40 x 10-6M, respectively. © 1969.

引用

页码：718 / &

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