INACTIVATION OF HIV-1 BY TRYPSIN AND ITS USE IN DEMONSTRATING SPECIFIC VIRUS-INFECTION OF CELLS

被引：49

作者：

TANG, SB

LEVY, JA

机构：

[1] UNIV CALIF SAN FRANCISCO,SCH MED,CANC RES INST,SAN FRANCISCO,CA 94143

[2] UNIV CALIF SAN FRANCISCO,SCH MED,DEPT MED,SAN FRANCISCO,CA 94143

来源：

JOURNAL OF VIROLOGICAL METHODS | 1991年 / 33卷 / 1-2期

关键词：

HIV; VIRUS INACTIVATION; TRYPSIN; CD4; RECEPTOR; SURFACE BINDING;

D O I：

10.1016/0166-0934(91)90005-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

HIV-1 was found sensitive to inactivation by low concentrations of trypsin. The use of trypsin was valuable for assessing non-specific binding of HIV virions to CD4+ cells. This effect was also helpful for eliminating input virus in experiments studying HIV infection of cells in vitro.

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页码：39 / 46

页数：8

相关论文

共 14 条

[1] HIV HETEROGENEITY AND VIRAL PATHOGENESIS [J].

CASTRO, BA ;

CHENGMAYER, C ;

EVANS, LA ;

LEVY, JA .

AIDS, 1988, 2 :S17-S27

[2] BIOLOGIC HETEROGENEITY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-2 (HIV-2) STRAINS [J].

CASTRO, BA ;

BARNETT, SW ;

EVANS, LA ;

MOREAU, J ;

ODEHOURI, K ;

LEVY, JA .

VIROLOGY, 1990, 178 (02) :527-534

[3] CHARACTERIZATION OF A NONCYTOPATHIC HIV-2 STRAIN WITH UNUSUAL EFFECTS ON CD4 EXPRESSION [J].

EVANS, LA ;

MOREAU, J ;

ODEHOURI, K ;

LEGG, H ;

BARBOZA, A ;

CHENGMAYER, C ;

LEVY, JA .

SCIENCE, 1988, 240 (4858) :1522-1525

[4] INFECTION OF HTLV-III/LAV IN HTLV-I-CARRYING CELLS MT-2 AND MT-4 AND APPLICATION IN A PLAQUE-ASSAY [J].

HARADA, S ;

KOYANAGI, Y ;

YAMAMOTO, N .

SCIENCE, 1985, 229 (4713) :563-566

[5] CHARACTERIZATION OF THE AIDS-ASSOCIATED RETROVIRUS REVERSE-TRANSCRIPTASE AND OPTIMAL CONDITIONS FOR ITS DETECTION IN VIRIONS [J].

HOFFMAN, AD ;

BANAPOUR, B ;

LEVY, JA .

VIROLOGY, 1985, 147 (02) :326-335

[6] AIDS-ASSOCIATED RETROVIRUSES (ARV) CAN PRODUCTIVELY INFECT OTHER CELLS BESIDES HUMAN T-HELPER CELLS [J].

LEVY, JA ;

SHIMABUKURO, J ;

MCHUGH, T ;

CASAVANT, C ;

STITES, D ;

OSHIRO, L .

VIROLOGY, 1985, 147 (02) :441-448

[7] LACK OF REQUIREMENT OF MURINE LEUKEMIA VIRUS FOR EARLY STEPS IN INFECTION OF MOUSE EMBRYO CELLS BY MURINE SARCOMA VIRUS [J].

LEVY, JA ;

ROWE, WP .

VIROLOGY, 1971, 45 (03) :844-&

[8] ROLE OF CD4 IN NORMAL IMMUNITY AND HIV INFECTION [J].

LIFSON, JD ;

ENGLEMAN, EG .

IMMUNOLOGICAL REVIEWS, 1989, 109 :93-117

[9]

MCDOUGAL JS, 1986, J IMMUNOL, V137, P2937

[10] DEXTRAN SULFATE SUPPRESSION OF VIRUSES IN THE HIV FAMILY - INHIBITION OF VIRION BINDING TO CD4+ CELLS [J].

MITSUYA, H ;

LOONEY, DJ ;

KUNO, S ;

UENO, R ;

WONGSTAAL, F ;

BRODER, S .

SCIENCE, 1988, 240 (4852) :646-649