STUDIES ON AQUEOUS POLYMER 2-PHASE SYSTEMS CONTAINING AGAROSE

被引：16

作者：

MEDIN, AS ^{[1
]}

JANSON, JC ^{[1
]}

机构：

[1] PHARMACIA BIOPROC TECHNOL AB,S-75182 UPPSALA,SWEDEN

来源：

CARBOHYDRATE POLYMERS | 1993年 / 22卷 / 02期

关键词：

D O I：

10.1016/0144-8617(93)90075-F

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Methods for high temperature preparation and separation of aqueous polymer two-phase systems containing agarose have been developed. A number of water-soluble polymers have been screened for polymer incompatibility and two-phase formation together with agarose. A method for analysis of the polymer phase composition based on chromatography at 70 degrees C on Superose 12((R)), a highly cross-linked, 10 mu m diameter agarose gel filtration medium, is presented. Phase diagrams for three agarose-poly(ethylene glycol) (PEG) and three agarose-dextran two-phase systems are described.

引用

页码：127 / 136

页数：10

共 16 条

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