DETERMINATION OF IVERMECTIN IN BOVINE PLASMA BY COLUMN-SWITCHING LC USING ONLINE SOLID-PHASE EXTRACTION AND TRACE ENRICHMENT

被引:15
作者
FISCHER, J [1 ]
KELLY, MT [1 ]
SMYTH, MR [1 ]
JANDERA, P [1 ]
机构
[1] UNIV CHEM TECHNOL PARDUBICE, DEPT ANALYT CHEM, PARDUBICE, CZECHOSLOVAKIA
关键词
IVERMECTIN; PLASMA LEVELS; LC; ONLINE SOLID-PHASE EXTRACTION; COLUMN SWITCHING;
D O I
10.1016/0731-7085(93)80200-K
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Ivermectin is a potent avermectin anthelmintic, derived from the soil organism Streptomyces avermitilis. Because of its potency, it is administered in very low doses (200 mug kg-1), giving rise to plasma levels in the ppb region, and hence sensitive methods are required for its determination in biological samples. A method is described for the determination of ivermectin in bovine plasma, based on a commercially available C18 column employing a mobile phase composed of acetonitrile-water-isopropanol (40:40:35, v/v/v). Extraction from plasma is carried out on-line using coupled-column technology; switching between the extraction and analytical columns is achieved using a six-port column-switching valve. The method is linear over the range 2-100 ng ml-1 (r = 0.9994) and has a limit of detection of 0.8 ng ml-1 (signal-to-noise ratio = 3). Reproducibility of the method, expressed as the overall mean relative standard deviation, is 6.7% (intra-assay) and 3.8% (inter-assay). The mean recovery of the drug from plasma is 76.4%. The method is simple and economical in its execution and is sufficiently reproducible to obviate the need for an internal standard.
引用
收藏
页码:217 / 223
页数:7
相关论文
共 17 条
[1]  
Alvernie M., 1987, ANN RECH VET, V18, P269
[2]  
ANDERSON RR, 1984, COMP CONT EDUC PRACT, V6, pS516
[3]  
BARRAGRY TB, 1987, CAN VET J, V28, P512
[4]   IVERMECTIN - A POTENT NEW ANTI-PARASITIC AGENT [J].
CAMPBELL, WC ;
FISHER, MH ;
STAPLEY, EO ;
ALBERSSCHONBERG, G ;
JACOB, TA .
SCIENCE, 1983, 221 (4613) :823-828
[5]   DETERMINATION OF IVERMECTIN RESIDUE IN ANIMAL-TISSUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY REVERSE ISOTOPE-DILUTION ASSAY [J].
CHIU, SHL ;
BUHS, RP ;
SESTOKAS, E ;
TAUB, R ;
JACOB, TA .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1985, 33 (01) :99-102
[6]   IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR QUANTITATION OF INVERMECTIN IN WHOLE-BLOOD, SERUM OR MUSCLE-TISSUE [J].
DICKINSON, CM .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 528 (01) :250-257
[7]   DETERMINATION OF IVERMECTIN IN FEEDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
FOX, A ;
FINK, DW .
ANALYST, 1985, 110 (03) :259-261
[8]   DETERMINATION OF 22,23-DIHYDROAVERMECTIN-B1A IN DOG PLASMA USING SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
KOJIMA, K ;
YAMAMOTO, K ;
NAKANISHI, Y ;
KATAE, H .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 413 :326-331
[9]   DETERMINATION OF IVERMECTIN RESIDUES IN SWINE TISSUES - AN IMPROVED CLEANUP PROCEDURE USING SOLID-PHASE EXTRACTION [J].
NORDLANDER, I ;
JOHNSSON, H .
FOOD ADDITIVES AND CONTAMINANTS, 1990, 7 (01) :79-82
[10]  
OEHLER DD, 1989, J ASSOC OFF ANA CHEM, V72, P59