CONVERSION OF BENZYL PENICILLIN TO 6-AMINOPENICILLANIC ACID USING AN INSOLUBLE DERIVATIVE OF PENICILLIN AMIDASE

被引：94

作者：

SELF, DA

KAY, G

LILLY, MD

DUNNILL, P

机构：

[1] Biochemical Engineering Section, Department of Chemical Engineering, University college, London

[2] Department of Botany, University College, London

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1969年 / 11卷 / 03期

关键词：

D O I：

10.1002/bit.260110306

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Penicillin amidase was extracted from Escherichia coli ATCC 9637, grown on phenylacetic, acid and glutamate, and purified by fractional ion with streptomycin sulphate, ammonium sulphate and polyethylene glycol, followed by chromatography on DEAE–cellulose. The purification factor was 100–200 × and the overall yield was about 115%. The enzyme was chemically attached to derivatives of cellulose and the kinetics of these insolubilized penicillin amidase preparations was investigated. Copyright © 1969 John Wiley & Sons, Inc.

引用

页码：337 / &

共 21 条

[1] 6-AMINOPENICILLANIC ACID .6. FORMATION OF 6-AMINOPENICILLANIC ACID FROM PENICILLIN BY ENZYMIC HYDROLYSIS
BATCHELOR, F
CHAIN, EB
RICHARDS, M
ROLINSON, GN
[J]. PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1961, 154 (957): : 522 - +
[2] 6-AMINOPENICILLANIC ACID .3. ISOLATION AND PURIFICATION
BATCHELOR, F
HARDY, TL
CHAIN, EB
ROLINSON, GN
MANSFORD, KR
[J]. PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1961, 154 (957): : 498 - +
[3] BACTERIAL PENICILLIN AMIDASE
CLARIDGE, CA
GOUREVITCH, A
LEIN, J
[J]. NATURE, 1960, 187 (4733) : 237 - 238
[4] CLARIDGE CA, 1963, P SOC EXP BIOL MED, V113, P1008, DOI 10.3181/00379727-113-28559
[5] FORMATION OF 6-AMINOPENICILLANIC ACID PENICILLINS AND PENICILLIN ACYLASE BY VARIOUS FUNGI
COLE, M
[J]. APPLIED MICROBIOLOGY, 1966, 14 (01) : 98 - &
[6] PREPARATION AND PROPERTIES OF FICIN CHEMICALLY ATTACHED TO CARBOXYMETHYLCELLULOSE
HORNBY, WE
LILLY, MD
CROOK, EM
[J]. BIOCHEMICAL JOURNAL, 1966, 98 (02) : 420 - &
[7] HUANG HT, 1963, APPL MICROBIOL, V11, P1
[8] ENZYMATISCHE SPALTUNG UND RESYNTHESE VON PENICILLIN
KAUFMANN, W
BAUER, K
[J]. NATURWISSENSCHAFTEN, 1960, 47 (20) : 474 - 475
[9] KAUFMANN W, 1964, J GEN MICROBIOL, V35, pR4
[10] Lilly M.D., 1968, CHEM ENG, V215, pCE12

← 1 2 3 →