IMPAIRED GENERATION OF PROSTAGLANDINS FROM ISOLATED GASTRIC SURFACE EPITHELIAL-CELLS IN PORTAL HYPERTENSIVE RATS

被引:16
作者
ARAKAWA, T [1 ]
TARNAWSKI, A [1 ]
MACH, T [1 ]
KOBAYASHI, K [1 ]
SARFEH, IJ [1 ]
机构
[1] UNIV CALIF IRVINE,DEPT MED & SURG,IRVINE,CA 92717
来源
PROSTAGLANDINS | 1990年 / 40卷 / 04期
关键词
D O I
10.1016/0090-6980(90)90102-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We studied generation of prostaglandins E2 and 6-keto F1a by surface epithelial cells isolated from the gastric mucosa of portal hypertensive and sham-operated rats. Oxygenated cell suspensions containing 80 ± 3% of surface epithelial cells were incubated for 30 min at 37°C and the concentration of prostaglandin E2 and 6-keto-prostaglandin F1a in medium was measured by radioimmunoassay. Viability of the cells was assessed with Fact green exclusion at baseline and after 30-min and 60-min incubation. Within 30 minutes the surface epithelial cells obtained from portal hypertensive rats generated 22.0 ± 1.6 (mean ± SE) pg prostaglandin E2 and 40.7 ± 4.7 pg 6-keto prostaglandin F1a, per 106 cells. These were significantly less than prostaglandin generation by cells obtained from sham-operated rats. The viability of the surface epithelial cells from portal hypertensive rats was also significantly reduced compared with sham-operated rats after 60 minutes incubation. Reduced abilityof the surface epithelial cells to generate prostaglandins may be one mechanism for increased susceptibility of portal hypertensive gastric mucosa to injury by noxious agents. © 1990.
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收藏
页码:373 / 382
页数:10
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