THE STRUCTURE OF PLANT-CELL WALLS .35. CHARACTERIZATION OF 7 XYLOGLUCAN OLIGOSACCHARIDES CONTAINING FROM 17 TO 20 GLYCOSYL RESIDUES

被引:47
作者
HISAMATSU, M [1 ]
YORK, WS [1 ]
DARVILL, AG [1 ]
ALBERSHEIM, P [1 ]
机构
[1] UNIV GEORGIA,COMPLEX CARBOHYDRATE RES CTR,220 RIVERBEND RD,ATHENS,GA 30602
关键词
D O I
10.1016/0008-6215(92)85060-D
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The complete primary structures of seven oligosaccharide subunits of the xyloglucan secreted by suspension-cultured Acer pseudoplatanus cells were determined. The oligosaccharides, ranging in size from 17 to 20 glycosyl residues, were generated by treatment of the xyloglucan with an endo-beta-(1 --> 4)-glucanase. The oligosaccharide components of a fraction obtained by Bio-Gel P-2 chromatography of enzyme-treated xyloglucan were further purified by normal-phase h.p.l.c. and then converted to the corresponding oligoglycosyl alditols by reduction with NaBH4. The oligoglycosyl alditols, after purification to near homogeneity by reversed-phase h.p.l.c., were structurally characterized by H-1-n.m.r. spectroscopy, fast-atom bombardment mass spectrometry (f.a.b.-m.s.), and analysis of their glycosyl-residue and glycosyl-linkage compositions. Novel structural elements of xyloglucans were observed in this study, including beta-D-xylopyranosyl and alpha-L-arabinofuranosyl-(1 --> 3)-beta-D-xylopyranosyl sidechains. The results also extend our list of correlations between H-1-n.m.r. resonances and specific structural features of xyloglucans and thus enhance our ability to determine the structures of xyloglucans from various sources.
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页码:45 / 71
页数:27
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