CYTOCHROME-P-450(17-ALPHA,LYASE)-MEDIATING PATHWAY OF ANDROGEN SYNTHESIS IN BOVINE ADRENOCORTICAL CULTURED-CELLS

被引：19

作者：

YAMAZAKI, T ^{[1
]}

NAWA, K ^{[1
]}

KOMINAMI, S ^{[1
]}

TAKEMORI, S ^{[1
]}

机构：

[1] HIROSHIMA UNIV,FAC INTEGRATED ARTS & SCI,NAKA KU,HIROSHIMA 730,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1134卷 / 02期

关键词：

ANDROGEN SYNTHESIS; PRIMARY CELL CULTURE; BOVINE ADRENAL; CYTOCHROME-P-450(17-ALPHA; LYASE); STEROIDOGENESIS;

D O I：

10.1016/0167-4889(92)90037-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytochrome P-450(17-alpha,lyase) mediating pathway of dehydroepiandrosterone (DHA) formation from pregnenolone was investigated in primary cultures of bovine adrenocortical fasciculata-reticularis cells. To determine whether DHA formation proceeds predominantly by successive monooxygenase reactions without 17-alpha-hydroxypregnenolone leaving P-450(17-alpha,lyase) the cells were incubated with [C-14]pregnenolone and 17-alpha-[H-3]hydroxypregnenolone in the presence of Trilostane. Results of the double-substrate double-label experiments indicate that in the presence of high concentration of pregnenolone most of DHA was formed 17-alpha-hydroxypregnenolone in the adrenal glands, DHA is concluded to be formed predominantly by successive reactions from pregnenolone without 17-alpha-hydroxypregnenolone leaving P-450(17-alpha,lyase) in vivo. By chronic ACTH treatment, the activities of 17-alpha-hydroxylation and DHA formation in adrenocortical cultured cells became higher concomitantly with the increase of P-450(17-alpha,lyase) content. Most of DHA was found to be formed by successive reactions from pregnenolone even under such conditions.

引用

页码：143 / 148

页数：6

共 24 条

[1]

AOYAMA Y, 1987, J BIOL CHEM, V262, P1239

[2] REGULATION OF SYNTHESIS AND ACTIVITY OF BOVINE ADRENOCORTICAL NADPH-CYTOCHROME-P-450 REDUCTASE BY ACTH [J].

DEE, A ;

CARLSON, G ;

SMITH, C ;

MASTERS, BS ;

WATERMAN, MR .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 128 (02) :650-656

[3]

FRIGUET B, 1989, PROTEIN STRUCTURE PR, P287

[4] KINETIC CONTROL OF STEROIDOGENESIS BY STEROID CONCENTRATION IN GUINEA-PIG ADRENAL MICROSOMES [J].

HIGUCHI, A ;

KOMINAMI, S ;

TAKEMORI, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1084 (03) :240-246

[5] THE CATALYTIC CYCLE OF CYTOCHROME-P-450SCC AND INTERMEDIATES IN THE CONVERSION OF CHOLESTEROL TO PREGNENOLONE [J].

HUME, R ;

KELLY, RW ;

TAYLOR, PL ;

BOYD, GS .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 140 (03) :583-591

[6] ADRENAL CYTOCHROME-P-450-11-BETA-PROTEOLIPOSOMES CATALYZING ALDOSTERONE SYNTHESIS - PREPARATION AND CHARACTERIZATION [J].

IKUSHIRO, S ;

KOMINAMI, S ;

TAKEMORI, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 984 (01) :50-56

[7]

Ingelman-Sundberg M, 1986, CYTOCHROME P450 STRU, P119

[8] AROMATIZATION OF DELTA-4-ANDROSTENE-3,17-DIONE, 19-HYDROXY-DELTA-4-ANDROSTENE-3,17-DIONE, AND 19-OXO-DELTA-4-ANDROSTENE-3,17-DIONE AT A COMMON CATALYTIC SITE IN HUMAN PLACENTAL MICROSOMES [J].

KELLY, WG ;

JUDD, D ;

STOLEE, A .

BIOCHEMISTRY, 1977, 16 (01) :140-145

[9] PURIFICATION AND SOME PROPERTIES OF CYTOCHROME-P-450 SPECIFIC FOR STEROID 17-ALPHA-HYDROXYLATION AND C17-C20 BOND-CLEAVAGE FROM GUINEA-PIG ADRENAL MICROSOMES [J].

KOMINAMI, S ;

SHINZAWA, K ;

TAKEMORI, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 109 (03) :916-921

[10] STEROIDOGENESIS IN LIPOSOMAL SYSTEM CONTAINING ADRENAL MICROSOMAL CYTOCHROME-P-450 ELECTRON-TRANSFER COMPONENTS [J].

KOMINAMI, S ;

INOUE, S ;

HIGUCHI, A ;

TAKEMORI, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 985 (03) :293-299

← 1 2 3 →