GERM LINE TRANSMISSION OF A YEAST ARTIFICIAL CHROMOSOME SPANNING THE MURINE ALPHA(1)(I) COLLAGEN LOCUS

被引：106

作者：

STRAUSS, WM

DAUSMAN, J

BEARD, C

JOHNSON, C

LAWRENCE, JB

JAENISCH, R

机构：

[1] MIT, DEPT BIOL, CAMBRIDGE, MA 02142 USA

[2] UNIV MASSACHUSETTS, SCH MED, WORCESTER, MA 01655 USA

来源：

SCIENCE | 1993年 / 259卷 / 5103期

关键词：

D O I：

10.1126/science.8096090

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Molecular complementation of mutant phenotypes by transgenic technology is a potentially important tool for gene identification. A technology was developed that allows the transfer of a physically intact yeast artificial chromosome (YAC) into the germ line of the mouse. A purified 150-kilobase YAC encompassing the murine gene Col1a1 was efficiently introduced into embryonic stem (ES) cells via lipofection. Chimeric founder mice were derived from two transfected ES cell clones. These chimeras transmitted the full length transgene through the germ line, generating two transgenic mouse strains. Transgene expression was visualized as nascent transcripts in interphase nuclei and quantitated by ribonuclease protection analysis. Both assays indicated that the transgene was expressed at levels comparable to the endogenous collagen gene.

引用

页码：1904 / 1907

页数：4

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