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IDENTIFICATION OF THE RON GENE-PRODUCT AS THE RECEPTOR FOR THE HUMAN MACROPHAGE STIMULATING PROTEIN

被引：240
作者：
WANG, MH
RONSIN, C
GESNEL, MC
COUPEY, L
SKEEL, A
LEONARD, EJ
BREATHNACH, R
机构：
[1] CHR NANTES,INST BIOL,INSERM,U211,F-44035 NANTES 01,FRANCE
[2] FAC MED LIMOGES,HEMATOL EXPTL LAB,F-87025 LIMOGES,FRANCE
来源：
SCIENCE | 1994年 / 266卷 / 5182期
关键词：
D O I：
10.1126/science.7939629
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Macrophage-stimulating protein (MSP) is a member of the hepatocyte growth factor-scatter factor (HGF-SF) family. Labeled MSP bound to Madin-Darby canine kidney (MDCK) cells transfected with complementary DNA encoding Ron, a cell membrane protein tyrosine kinase. Cross-lin king of I-125-labeled MSP to transfected cells (MDCK-RE7 cells) and immunoprecipitation by antibodies to Ron revealed a 220-kilodalton complex, a size consistent with that of MSP (80 kilodaltons) cross-linked to the beta chain of Ron (150 kilodaltons). The binding of I-125-labeled MSP to MDCK-RE7 cells was inhibited by unlabeled MSP, but not by HGF-SF. MSP caused phosphorylation of the beta chain of Ron and induced migration of MDCK-RE7 cells. These results establish the ron gene product as a specific cell-surface receptor tor MSP.
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页码：117 / 119
页数：3
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