MOLECULAR-CLONING AND TRANSCRIPTIONAL ANALYSIS OF THE CPEBA OPERON OF THE CYANOBACTERIUM PSEUDANABAENA SPECIES PCC-7409

被引：50

作者：

DUBBS, JM ^{[1
]}

BRYANT, DA ^{[1
]}

机构：

[1] PENN STATE UNIV, DEPT MOLEC & CELL BIOL, University Pk, PA 16802 USA

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 12期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb01867.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cpeBA operon of the Group III chromatically adapting cyanobacterium Pseudanabaena species PCC 7409 was cloned, sequenced and characterized. The cpeBA genes are transcribed in green-light-grown cells as an abundant 1400-nucleotide mRNA which initiates 69 nucleotides upstream from the cpeB translation start. Extensive sequence identity, extending 70 nucleotides 5' to the transcription start, occurs among cpeBA promoters of Group II and III chromatic adapters. Cell extracts of green-light-grown Calothrix species PCC 7601 contain an activity which specifically binds a restriction fragment containing the Pseudanabaena species PCC 7409 cpeBA promoter. Green-light-dependent cpeBA transcription in Group II and III chromatically adapting cyanobacteria is suggested to be similarly controlled by a transcriptional activator.

引用

页码：3073 / 3085

页数：13

共 67 条

[1] STRUCTURE AND LIGHT-REGULATED EXPRESSION OF PHYCOERYTHRIN GENES IN WILD-TYPE AND PHYCOBILISOME ASSEMBLY MUTANTS OF SYNECHOCYSTIS SP STRAIN PCC-6701 [J].

ANDERSON, LK ;

GROSSMAN, AR .

JOURNAL OF BACTERIOLOGY, 1990, 172 (03) :1297-1305

[2]

Ausubel FM, 1987, CURRENT PROTOCOLS MO, V1-5

[3] PHYCOBILIPROTEINS AND COMPLEMENTARY CHROMATIC ADAPTATION [J].

BOGORAD, L .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1975, 26 :369-401

[4]

Bryant D.A., 1988, LIGHT ENERGY TRANSDU, P62

[5]

Bryant D.A., 1991, CELL CULTURE SOMATIC, V1st ed., P255

[6]

BRYANT DA, 1982, J GEN MICROBIOL, V128, P835

[7]

BRYANT DA, 1988, METHOD ENZYMOL, V167, P755

[8] EFFECTS OF CHROMATIC ILLUMINATION ON CYANOBACTERIAL PHYCOBILISOMES - EVIDENCE FOR THE SPECIFIC INDUCTION OF A 2ND PAIR OF PHYCOCYANIN SUBUNITS IN PSEUDOANABAENA-7409 GROWN IN RED-LIGHT [J].

BRYANT, DA ;

COHENBAZIRE, G .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1981, 119 (02) :415-424

[9] A SMALL MULTIGENE FAMILY ENCODES THE ROD-CORE LINKER POLYPEPTIDES OF ANABAENA SP PCC7120 PHYCOBILISOMES [J].

BRYANT, DA ;

STIREWALT, VL ;

GLAUSER, M ;

FRANK, G ;

SIDLER, W ;

ZUBER, H .

GENE, 1991, 107 (01) :91-99

[10] THE PHOTOREGULATED EXPRESSION OF MULTIPLE PHYCOCYANIN SPECIES - A GENERAL MECHANISM FOR THE CONTROL OF PHYCOCYANIN SYNTHESIS IN CHROMATICALLY ADAPTING CYANOBACTERIA [J].

BRYANT, DA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1981, 119 (02) :425-429

← 1 2 3 4 5 6 7 →