SYNTHESIS AND CHARACTERIZATION OF OLIGODEOXYNUCLEOTIDES CONTAINING THE MUTAGENIC BASE ANALOG 4-O-ETHYLTHYMINE

被引:29
作者
FERNANDEZFORNER, D
PALOM, Y
IKUTA, S
PEDROSO, E
ERITJA, R
机构
[1] UNIV BARCELONA,DEPT ORGAN CHEM,MARTI & FRANQUES 1-11,E-08028 BARCELONA,SPAIN
[2] CTR RES & DEV,DEPT MOLEC GENET,E-08034 BARCELONA,SPAIN
[3] IIT,DEPT CHEM,CHICAGO,IL 60616
关键词
D O I
10.1093/nar/18.19.5729
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A method for the preparation of oligonucleotides containing the mutagenic base 4-O-ethyHhymine is described for the first time. Use of p-nitrophenylethyl type base protecting groups together with phosphite-triester solid-phase methodology makes possible the rapid and efficient preparation of oligonucleotides bearing 4-O-ethylthymine, while standard base protecting groups are not compatible with the presence of this base. Possible applications of this methodology are discussed. © 1990 Oxford University Press.
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页码:5729 / 5734
页数:6
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