SYNTHESIS AND CHARACTERIZATION OF OLIGODEOXYNUCLEOTIDES CONTAINING THE MUTAGENIC BASE ANALOG 4-O-ETHYLTHYMINE

被引：29

作者：

FERNANDEZFORNER, D

PALOM, Y

IKUTA, S

PEDROSO, E

ERITJA, R

机构：

[1] UNIV BARCELONA,DEPT ORGAN CHEM,MARTI & FRANQUES 1-11,E-08028 BARCELONA,SPAIN

[2] CTR RES & DEV,DEPT MOLEC GENET,E-08034 BARCELONA,SPAIN

[3] IIT,DEPT CHEM,CHICAGO,IL 60616

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 19期

关键词：

D O I：

10.1093/nar/18.19.5729

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the preparation of oligonucleotides containing the mutagenic base 4-O-ethyHhymine is described for the first time. Use of p-nitrophenylethyl type base protecting groups together with phosphite-triester solid-phase methodology makes possible the rapid and efficient preparation of oligonucleotides bearing 4-O-ethylthymine, while standard base protecting groups are not compatible with the presence of this base. Possible applications of this methodology are discussed. © 1990 Oxford University Press.

引用

页码：5729 / 5734

页数：6

共 18 条

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