NITROGENASE CONFINED TO RANDOMLY DISTRIBUTED TRICHOMES IN THE MARINE CYANOBACTERIUM TRICHODESMIUM-THIEBAUTII

被引：71

作者：

BERGMAN, B ^{[1
]}

CARPENTER, EJ ^{[1
]}

机构：

[1] SUNY STONY BROOK,MARINE SCI RES CTR,STONY BROOK,NY 11794

来源：

JOURNAL OF PHYCOLOGY | 1991年 / 27卷 / 02期

关键词：

CYANOBACTERIUM; IMMUNOLOCALIZATION; NITROGENASE; NITROGEN FIXATION; OSCILLATORIA; TEM; TRICHODESMIUM;

D O I：

10.1111/j.0022-3646.1991.00158.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Nitrogenase reductase (Fe-protein) was detected in the marine planktonic cyanobacterium Trichodesmium. The molecular weight was about 38 kD, as shown by western blotting using anti-Rhodospirillum rubrum nitrogenase reductase antiserum. The enzyme was confined to a limited number (ca. 10-40%) of randomly distributed trichomes in the Trichodesmium colonies, as shown by immunogold localization and transmission electron microscopy. Associated microorganisms had little or no nitrogenase. Nitrogenase showed a diel cycle in localization: present throughout the cytoplasm of cells in N2-fixing (daytime) colonies but at the periphery of non-N2-fixing (nighttime) colonies. This structural arrangement of N2-fixing trichomes and nitrogenase is novel and different from the previously held paradigm for this and other diazotrophic cyanobacteria.

引用

页码：158 / 165

页数：8

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