AUTOMATIC CELLULASE ASSAY IN COMPUTER COUPLED PILOT FERMENTATION

被引：6

作者：

LEISOLA, M

VIRKKUNEN, J

KARVONEN, E

MESKANEN, A

机构：

[1] HELSINKI UNIV TECHNOL,DEPT ELECT ENGN,SF-02150 ESPOO 15,FINLAND

[2] TECH RES CTR FINLAND,BIOTECH LAB,SF-00121 HELSINKI 12,FINLAND

[3] RINTEKNO OY,SF-02620 ESPOO 62,FINLAND

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1979年 / 1卷 / 02期

关键词：

D O I：

10.1016/0141-0229(79)90109-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Equipment for the automatic measurement of cellulase activity was connected to a computer-coupled pilot fermenter. The measurement was based on the use of dyed Avicel cellulose as substrate. An intermittent sampling device was used to take a sample from the fermenter at intervals of one hour. The computer was programmed to calculate the cellulase activity during fermentation. The measured activity values were corrected against a standard sample of known activity by means of a mathematical model for the calibration curve which was stored in the computer. On-line measurement of enzyme activity was successfully performed. © 1979.

引用

页码：117 / 121

页数：5

共 7 条

[1] AUTOMATIC ASSAY OF CELLULASE ACTIVITY DURING FERMENTATION
LEISOLA, M
KAUPPINEN, V
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1978, 20 (06) : 837 - 846
[2] LEISOLA M, 1977, FINN CHEM LETT, V6, P172
[3] LUNDELL R, 1976, PROCESS BIOCHEM, V11, P13
[4] LUNDELL R, 1976, 166TH EUR FED CHEM E
[5] MESKANEN A, 1976, PROCESS BIOCHEM, V11, P31
[6] AUTOMATIC DETERMINATION OF GLUCOSE, ETHANOL, AMINO NITROGEN, AND AMMONIA - CELL COUNTING AND DATA-PROCESSING
MOR, J
ZIMMERLI, A
FIECHTER, A
[J]. ANALYTICAL BIOCHEMISTRY, 1973, 52 (02) : 614 - 624
[7] RAPID SAMPLING OF YEAST-CELLS AND AUTOMATED ASSAYS OF ADENYLATE, CITRATE, PYRUVATE AND GLUCOSE-6-PHOSPHATE POOLS
WEIBEL, KE
MOR, JR
FIECHTER, A
[J]. ANALYTICAL BIOCHEMISTRY, 1974, 58 (01) : 208 - 216

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