CYTOPLASMIC DYNEIN IS REQUIRED FOR NORMAL NUCLEAR SEGREGATION IN YEAST

被引：323

作者：

ESHEL, D

URRESTARAZU, LA

VISSERS, S

JAUNIAUX, JC

VANVLIETREEDIJK, JC

PLANTA, RJ

GIBBONS, IR

机构：

[1] UNIV LIBRE BRUXELLES, PHYSIOL CELLULAIRE & GENET LEVURES LAB, B-1050 BRUSSELS, BELGIUM

[2] DEUTSCH KREBSFORSCHUNGSZENTRUM, FORSCHUNGSSCHWERPUNKT ANGEW TUMORVIROL, W-6900 HEIDELBERG, GERMANY

[3] VRIJE UNIV AMSTERDAM, BIOCHEM LAB, AMSTERDAM 1081, NETHERLANDS

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 23期

关键词：

MOTOR PROTEIN; CYTOPLASMIC MICROTUBULES; NUCLEAR DIVISION; MITOSIS;

D O I：

10.1073/pnas.90.23.11172

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have identified the gene DYN1, which encodes the heavy chain of cytoplasmic dynein in the yeast Saccharomyces cerevisiae. The predicted amino acid sequence (M(r) 471,305) reveals the presence of four P-loop motifs, as in all dyneins known so far, and has 28% overall identity to the dynein heavy chain of Dictyostelium [Koonce, M. P., Grissom, P. M. & McIntosh, J. R. (1992) J. Cell Biol. 119, 1597-1604] with 40% identity in the putative motor domain. Disruption of DYN1 causes misalignment of the spindle relative to the bud neck during cell division and results in abnormal distribution of the dividing nuclei between the mother cell and the bud. Cytoplasmic dynein, by generating force along cytoplasmic microtubules, may play an important role in the proper alignment of the mitotic spindle in yeast.

引用

页码：11172 / 11176

页数：5

共 29 条

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