EFFECTS OF NICARDIPINE ON TUBE FORMATION OF BOVINE VASCULAR ENDOTHELIAL-CELLS INVITRO

被引：13

作者：

KANEKO, T ^{[1
]}

NAGATA, I ^{[1
]}

MIYAMOTO, S ^{[1
]}

KUBO, H ^{[1
]}

KIKUCHI, H ^{[1
]}

FUJISATO, T ^{[1
]}

IKADA, Y ^{[1
]}

机构：

[1] KYOTO UNIV,BIOMED ENGN RES CTR,KYOTO 606,JAPAN

来源：

STROKE | 1992年 / 23卷 / 11期

关键词：

ANGIOGENESIS; ENDOTHELIUM; NICARDIPINE; CATTLE;

D O I：

10.1161/01.STR.23.11.1637

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Background and Purpose: The purpose of this study was to assess the effect of nicardipine, a Ca2+ channel blocker, on angiogenesis in vitro. Methods: Bovine carotid artery endothelial cells were cultured between type I collagen gel layers with 10(-9) to 10(-5) M nicardipine. The morphological changes were monitored by phase-contrast microscopy and photographed. The total length of tubular structures was measured with an image analyzer system. Endothelial proliferation and migration assays were also performed with the same doses of nicardipine. Results: Cultured endothelial cells form tubular structures between collagen gel layers. Tube formation of endothelial cells was suppressed by culture with 10(-9) to 10(-5) M nicardipine in a dose-dependent manner. Migration of endothelial cells was also suppressed by the same doses of nicardipine. However, proliferation of endothelial cells was not enhanced. Conclusions: Nicardipine acts as an inhibitor of angiogenesis in vitro by inhibiting the migration of endothelial cells. This result suggests that nicardipine may have therapeutic potential in angiogenic disorders such as tumor growth, atherogenesis, and diabetic retinopathy.

引用

页码：1637 / 1642

页数：6

共 31 条

[1] MIGRATION AND PROLIFERATION OF ENDOTHELIAL CELLS IN PREFORMED AND NEWLY FORMED BLOOD-VESSELS DURING TUMOR ANGIOGENESIS [J].