ISOLATION OF A NOVEL SULPHATASE FROM RAT LIVER

被引：29

作者：

TUDBALL, N

DAVIDSON, EA

机构：

[1] Department of Biochemistry, Duke University Medical Center, Durham, NC

[2] Department of Biological Chemistry, Pennsylvania State University College of Medicine, Hershey Medical Center, Hershey, PA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 171卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0005-2744(69)90110-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A lysosomal sulphatase active towards an oligosaccharide sulphate, has been isolated from rat liver and partially purified. The purified system still contained β-glucuronidase, β-N-acetylhexosaminidase and arylsulphatase activities. The substrate employed for the assay was isolated from the digestion products obtained from chick embryo chondroitin sulphate A following testicular hyaluronidase digestion. Specificity studies indicated that the system was only active towards relatively high molecular weight substrates, but not towards chondroitin sulphate A itself. Prior cleavage of the nonterminal glucuronosyl residue of the oligosaccharide was apparently essential in the overall hydrolytic process. © 1969.

引用

页码：113 / &

共 14 条

[1]

ARONSON NN, 1967, J BIOL CHEM, V242, P437

[2]

ARONSON NN, 1968, J BIOL CHEM, V243, P4494

[3]

COLOWICK SP, 1966, METHODS ENZYMOLOG ED, V8, P663

[4]

COLOWICK SP, 1966, METHODS ENZYMOLOG ED, V8, P571

[5] STUDIES ON SULPHATASES .9. ARYLSULPHATASES OF MAMMALIAN LIVERS [J].